منابع مشابه
Hanging Drop Tissue Culture
The hanging drop tissue culture is a technique utilized in embryology [4] and other fields to allow growth that would otherwise be restricted by the flat plane of culture dishes and also to minimize the surface area to volume ratio, slowing evaporation. The classic hanging drop culture is a small drop of liquid, such as plasma or some other media allowing tissue growth, suspended from an invert...
متن کاملEtiology and Use of the “Hanging Drop” Technique: A Review
Background. The hanging drop (HD) technique presumably relies on the presence of subatmospheric epidural pressure. It is not clear whether this negative pressure is intrinsic or an artifact and how it is affected by body position. There are few data to indicate how often HD is currently being used. Methods. We identified studies that measured subatmospheric pressures and looked at the effect of...
متن کاملThe Hanging Drop Slide Technique to Study Bacterial Motility
In this exercise you will learn to make hanging drop slides and then use this technique to observe the motility of living bacteria. Motility is an important characteristic used to identify microorganisms. Three bacteria will be used which vary in size, shape and arrangement of flagella, and types of motion. Specifically you will observe Pseudomonas aeruginosa, a monotrichous bacterium that exhi...
متن کاملCrystallization of Aspergillus japonicus lipase by hanging drop method
Lipase purified from Aspergillus japonicus isolated from Ropalidia marginata paper nest was attempted for crystallization by hanging drop method. 2-methyl2, 4-pentanediol (MPD) was used as the precipitating agent and single crystals of A. japonicus lipase were obtained by using protein concentrated to 10 mg/mL and a reservoir solution containing 10-20% MPD buffered with 150 mM cacodylate buffer...
متن کاملMicro ATR FTIR imaging of hanging drop protein crystallisation
Protein crystallisation is of great importance within structural proteomic projects where considerable time and effort is spent trying to obtain high resolution structural information from the X-ray diffraction of protein crystals. The crystallisation process is largely empirical due to a lack of understanding of crystallisation kinetics and it is often very challenging to obtain crystals of su...
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ژورنال
عنوان ژورنال: Anaesthesia
سال: 1953
ISSN: 0003-2409,1365-2044
DOI: 10.1111/j.1365-2044.1953.tb03119.x